Heat-stable enterotoxin receptor

UniProt data

UniProt name: GUC2C_HUMAN (P25092)

Gene name: GUCY2C; Synonyms=GUC2C, STAR

Organism: Homo sapiens (Human).

Function: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptides guanylin and uroguanylin.

Subcellular location: Cell membrane {ECO:0000269|PubMed:23269669}; Single-pass type I membrane protein {ECO:0000269|PubMed:23269669}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:23269669}; Single-pass type I membrane protein {ECO:0000269|PubMed:23269669}. Note=The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form.

Transmembrane domains:

  1. 431-454, 24 AA, Helical. {ECO:0000255}.

Sequence: 1073 AA

10 20 30 40 50 60 MKTLLLDLAL WSLLFQPGWL SFSSQVSQNC HNGSYEISVL MMGNSAFAEP LKNLEDAVNE 70 80 90 100 110 120 GLEIVRGRLQ NAGLNVTVNA TFMYSDGLIH NSGDCRSSTC EGLDLLRKIS NAQRMGCVLI 130 140 150 160 170 180 GPSCTYSTFQ MYLDTELSYP MISAGSFGLS CDYKETLTRL MSPARKLMYF LVNFWKTNDL 190 200 210 220 230 240 PFKTYSWSTS YVYKNGTETE DCFWYLNALE ASVSYFSHEL GFKVVLRQDK EFQDILMDHN 250 260 270 280 290 300 RKSNVIIMCG GPEFLYKLKG DRAVAEDIVI ILVDLFNDQY FEDNVTAPDY MKNVLVLTLS 310 320 330 340 350 360 PGNSLLNSSF SRNLSPTKRD FALAYLNGIL LFGHMLKIFL ENGENITTPK FAHAFRNLTF 370 380 390 400 410 420 EGYDGPVTLD DWGDVDSTMV LLYTSVDTKK YKVLLTYDTH VNKTYPVDMS PTFTWKNSKL 430 440 450 460 470 480 PNDITGRGPQ ILMIAVFTLT GAVVLLLLVA LLMLRKYRKD YELRQKKWSH IPPENIFPLE 490 500 510 520 530 540 TNETNHVSLK IDDDKRRDTI QRLRQCKYDK KRVILKDLKH NDGNFTEKQK IELNKLLQID 550 560 570 580 590 600 YYNLTKFYGT VKLDTMIFGV IEYCERGSLR EVLNDTISYP DGTFMDWEFK ISVLYDIAKG 610 620 630 640 650 660 MSYLHSSKTE VHGRLKSTNC VVDSRMVVKI TDFGCNSILP PKKDLWTAPE HLRQANISQK 670 680 690 700 710 720 GDVYSYGIIA QEIILRKETF YTLSCRDRNE KIFRVENSNG MKPFRPDLFL ETAEEKELEV 730 740 750 760 770 780 YLLVKNCWEE DPEKRPDFKK IETTLAKIFG LFHDQKNESY MDTLIRRLQL YSRNLEHLVE 790 800 810 820 830 840 ERTQLYKAER DRADRLNFML LPRLVVKSLK EKGFVEPELY EEVTIYFSDI VGFTTICKYS 850 860 870 880 890 900 TPMEVVDMLN DIYKSFDHIV DHHDVYKVET IGDAYMVASG LPKRNGNRHA IDIAKMALEI 910 920 930 940 950 960 LSFMGTFELE HLPGLPIWIR IGVHSGPCAA GVVGIKMPRY CLFGDTVNTA SRMESTGLPL 970 980 990 1000 1010 1020 RIHVSGSTIA ILKRTECQFL YEVRGETYLK GRGNETTYWL TGMKDQKFNL PTPPTVENQQ 1030 1040 1050 1060 1070 RLQAEFSDMI ANSLQKRQAA GIRSQKPRRV ASYKKGTLEY LQLNTTDKES TYF

CATM prediction

The sequence of GUC2C_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
    430       440       450     
  |    +    |    +    |    +  		 (Å) (°) (Å) (°)  
Model 1 GAQILMIAVFTLTGAVVLLLLVALLMLRKY 7.0 -53.7° 2.5 33.1 -15.7
Prolines* GPQILMIAVFTLTGAVVLLLLVALLMLRKY          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 438, THR, HA A, 437, PHE, O 3.40Å
B, 441, GLY, HA1 A, 438, THR, O 3.04Å
A, 438, THR, HA B, 437, PHE, O 3.40Å
A, 441, GLY, HA1 B, 438, THR, O 3.04Å