Myeloid cell surface antigen CD33

UniProt data

UniProt name: CD33_HUMAN (P20138)

Gene name: CD33; Synonyms=SIGLEC3

Organism: Homo sapiens (Human).

Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). {ECO:0000269|PubMed:10556798, ECO:0000269|PubMed:11320212}.

Subcellular location: Cell membrane; Single-pass type I membrane protein.

Transmembrane domains:

  1. 260-282, 23 AA, Helical. {ECO:0000255}.

Sequence: 364 AA

10 20 30 40 50 60 MPLLLLLPLL WAGALAMDPN FWLQVQESVT VQEGLCVLVP CTFFHPIPYY DKNSPVHGYW 70 80 90 100 110 120 FREGAIISRD SPVATNKLDQ EVQEETQGRF RLLGDPSRNN CSLSIVDARR RDNGSYFFRM 130 140 150 160 170 180 ERGSTKYSYK SPQLSVHVTD LTHRPKILIP GTLEPGHSKN LTCSVSWACE QGTPPIFSWL 190 200 210 220 230 240 SAAPTSLGPR TTHSSVLIIT PRPQDHGTNL TCQVKFAGAG VTTERTIQLN VTYVPQNPTT 250 260 270 280 290 300 GIFPGDGSGK QETRAGVVHG AIGGAGVTAL LALCLCLIFF IVKTHRRKAA RTAVGRNDTH 310 320 330 340 350 360 PTTGSASPKH QKKSKLHGPT ETSSCSGAAP TVEMDEELHY ASLNFHGMNP SKDTSTEYSE VRTQ

CATM prediction

The sequence of CD33_HUMAN returned 4 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
     260       270       280   
   |    +    |    +    |    +		 (Å) (°) (Å) (°)  
Model 1 VVHGAIGGAGVTALLALCLCLIFFIVKTH 6.6 -46.0° 4.7 38.2 -26.6
Model 2 VVHGAIGGAGVTALLALCLCLIFFIVKTH 6.4 -55.0° 4.9 53.2 -16.5
Model 3 VVHGAIGGAGVTALLALCLCLIFFIVKTH 7.1 +52.0° 1.8 1.0 -6.9
Model 4 VVHGAIGGAGVTALLALCLCLIFFIVKTH 6.8 -54.0° 2.5 33.0 -5.3

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 261, ALA, HA A, 260, GLY, O 2.48Å
B, 264, GLY, HA1 A, 261, ALA, O 2.69Å
B, 265, ALA, HA A, 268, THR, OG1 2.57Å
A, 261, ALA, HA B, 260, GLY, O 2.48Å
A, 264, GLY, HA1 B, 261, ALA, O 2.69Å
A, 265, ALA, HA B, 268, THR, OG1 2.57Å