Tyrosinase

UniProt data

UniProt name: TYRO_HUMAN (P14679)

Gene name: TYR

Organism: Homo sapiens (Human).

Function: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4- dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6- dihydroxyindole) to indole-5,6 quinone. {ECO:0000250|UniProtKB:P11344}.

Subcellular location: Melanosome membrane {ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065}; Single-pass type I membrane protein {ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065}. Melanosome {ECO:0000250|UniProtKB:P11344}. Note=Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. {ECO:0000250|UniProtKB:P11344}.

Transmembrane domains:

  1. 477-497, 21 AA, Helical. {ECO:0000255}.

Sequence: 529 AA

10 20 30 40 50 60 MLLAVLYCLL WSFQTSAGHF PRACVSSKNL MEKECCPPWS GDRSPCGQLS GRGSCQNILL 70 80 90 100 110 120 SNAPLGPQFP FTGVDDRESW PSVFYNRTCQ CSGNFMGFNC GNCKFGFWGP NCTERRLLVR 130 140 150 160 170 180 RNIFDLSAPE KDKFFAYLTL AKHTISSDYV IPIGTYGQMK NGSTPMFNDI NIYDLFVWMH 190 200 210 220 230 240 YYVSMDALLG GSEIWRDIDF AHEAPAFLPW HRLFLLRWEQ EIQKLTGDEN FTIPYWDWRD 250 260 270 280 290 300 AEKCDICTDE YMGGQHPTNP NLLSPASFFS SWQIVCSRLE EYNSHQSLCN GTPEGPLRRN 310 320 330 340 350 360 PGNHDKSRTP RLPSSADVEF CLSLTQYESG SMDKAANFSF RNTLEGFASP LTGIADASQS 370 380 390 400 410 420 SMHNALHIYM NGTMSQVQGS ANDPIFLLHH AFVDSIFEQW LRRHRPLQEV YPEANAPIGH 430 440 450 460 470 480 NRESYMVPFI PLYRNGDFFI SSKDLGYDYS YLQDSDPDSF QDYIKSYLEQ ASRIWSWLLG 490 500 510 520 AAMVGAVLTA LLAGLVSLLC RHKRKQLPEE KQPLLMEKED YHSLYQSHL

CATM prediction

The sequence of TYRO_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
        480       490       5
 +    |    +    |    +    |		 (Å) (°) (Å) (°)  
Model 1 IWSWLLGAAMVGAVLTALLAGLVSLLC 6.8 -46.0° 2.7 46.9 -31.0
Model 2 IWSWLLGAAMVGAVLTALLAGLVSLLC 7.3 -55.0° 2.4 48.0 -4.7

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 482, ALA, HA A, 481, ALA, O 2.89Å
B, 485, GLY, HA1 A, 482, ALA, O 2.92Å
B, 486, ALA, HA A, 489, THR, OG1 2.40Å
A, 482, ALA, HA B, 481, ALA, O 2.89Å
A, 485, GLY, HA1 B, 482, ALA, O 2.92Å
A, 486, ALA, HA B, 489, THR, OG1 2.40Å