Lysosome-associated membrane glycoprotein 2

UniProt data

UniProt name: LAMP2_HUMAN (P13473)

Gene name: LAMP2

Organism: Homo sapiens (Human).

Function: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. {ECO:0000269|PubMed:8407947}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:17897319}; Single-pass type I membrane protein {ECO:0000255|PROSITE- ProRule:PRU00740, ECO:0000269|PubMed:17897319}. Endosome membrane {ECO:0000269|PubMed:17897319}; Single-pass type I membrane protein {ECO:0000255|PROSITE-ProRule:PRU00740, ECO:0000269|PubMed:17897319}. Lysosome membrane {ECO:0000255|PROSITE-ProRule:PRU00740, ECO:0000269|PubMed:17897319}; Single-pass type I membrane protein {ECO:0000255|PROSITE-ProRule:PRU00740, ECO:0000269|PubMed:17897319}. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Transmembrane domains:

  1. 376-399, 24 AA, Helical. {ECO:0000255|PROSITE- ProRule:PRU00740}.

Sequence: 410 AA

10 20 30 40 50 60 MVCFRLFPVP GSGLVLVCLV LGAVRSYALE LNLTDSENAT CLYAKWQMNF TVRYETTNKT 70 80 90 100 110 120 YKTVTISDHG TVTYNGSICG DDQNGPKIAV QFGPGFSWIA NFTKAASTYS IDSVSFSYNT 130 140 150 160 170 180 GDNTTFPDAE DKGILTVDEL LAIRIPLNDL FRCNSLSTLE KNDVVQHYWD VLVQAFVQNG 190 200 210 220 230 240 TVSTNEFLCD KDKTSTVAPT IHTTVPSPTT TPTPKEKPEA GTYSVNNGND TCLLATMGLQ 250 260 270 280 290 300 LNITQDKVAS VININPNTTH STGSCRSHTA LLRLNSSTIK YLDFVFAVKN ENRFYLKEVN 310 320 330 340 350 360 ISMYLVNGSV FSIANNNLSY WDAPLGSSYM CNKEQTVSVS GAFQINTFDL RVQPFNVTQG 370 380 390 400 KYSTAQDCSA DDDNFLVPIA VGAALAGVLI LVLLAYFIGL KHHHAGYEQF

CATM prediction

The sequence of LAMP2_HUMAN returned 3 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
         380       390       400
  +    |    +    |    +    |  		 (Å) (°) (Å) (°)  
Model 1 DNFLVAIAVGAALAGVLILVLLAYFIGLKH 6.7 -51.2° 2.4 45.0 -38.8
Model 2 DNFLVAIAVGAALAGVLILVLLAYFIGLKH 7.1 -54.0° 4.8 40.7 -9.1
Model 3 DNFLVAIAVGAALAGVLILVLLAYFIGLKH 7.1 -43.0° 0.0 33.8 -3.3
Prolines* DNFLVPIAVGAALAGVLILVLLAYFIGLKH          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 379, ILE, HA A, 378, ALA, O 2.94Å
B, 382, GLY, HA1 A, 379, ILE, O 2.55Å
B, 383, ALA, HA A, 382, GLY, O 3.13Å
A, 379, ILE, HA B, 378, ALA, O 2.94Å
A, 382, GLY, HA1 B, 379, ILE, O 2.55Å
A, 383, ALA, HA B, 382, GLY, O 3.13Å