Thyroid peroxidase

UniProt data

UniProt name: PERT_HUMAN (P07202)

Gene name: TPO

Organism: Homo sapiens (Human).

Function: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). {ECO:0000250|UniProtKB:P09933}.

Subcellular location: Isoform 3: Cell surface.

Transmembrane domains:

  1. 847-871, 25 AA, Helical. {ECO:0000255}.

Sequence: 933 AA

10 20 30 40 50 60 MRALAVLSVT LVMACTEAFF PFISRGKELL WGKPEESRVS SVLEESKRLV DTAMYATMQR 70 80 90 100 110 120 NLKKRGILSP AQLLSFSKLP EPTSGVIARA AEIMETSIQA MKRKVNLKTQ QSQHPTDALS 130 140 150 160 170 180 EDLLSIIANM SGCLPYMLPP KCPNTCLANK YRPITGACNN RDHPRWGASN TALARWLPPV 190 200 210 220 230 240 YEDGFSQPRG WNPGFLYNGF PLPPVREVTR HVIQVSNEVV TDDDRYSDLL MAWGQYIDHD 250 260 270 280 290 300 IAFTPQSTSK AAFGGGADCQ MTCENQNPCF PIQLPEEARP AAGTACLPFY RSSAACGTGD 310 320 330 340 350 360 QGALFGNLST ANPRQQMNGL TSFLDASTVY GSSPALERQL RNWTSAEGLL RVHARLRDSG 370 380 390 400 410 420 RAYLPFVPPR APAACAPEPG IPGETRGPCF LAGDGRASEV PSLTALHTLW LREHNRLAAA 430 440 450 460 470 480 LKALNAHWSA DAVYQEARKV VGALHQIITL RDYIPRILGP EAFQQYVGPY EGYDSTANPT 490 500 510 520 530 540 VSNVFSTAAF RFGHATIHPL VRRLDASFQE HPDLPGLWLH QAFFSPWTLL RGGGLDPLIR 550 560 570 580 590 600 GLLARPAKLQ VQDQLMNEEL TERLFVLSNS STLDLASINL QRGRDHGLPG YNEWREFCGL 610 620 630 640 650 660 PRLETPADLS TAIASRSVAD KILDLYKHPD NIDVWLGGLA ENFLPRARTG PLFACLIGKQ 670 680 690 700 710 720 MKALRDGDWF WWENSHVFTD AQRRELEKHS LSRVICDNTG LTRVPMDAFQ VGKFPEDFES 730 740 750 760 770 780 CDSITGMNLE AWRETFPQDD KCGFPESVEN GDFVHCEESG RRVLVYSCRH GYELQGREQL 790 800 810 820 830 840 TCTQEGWDFQ PPLCKDVNEC ADGAHPPCHA SARCRNTKGG FQCLCADPYE LGDDGRTCVD 850 860 870 880 890 900 SGRLPRVTWI SMSLAALLIG GFAGLTSTVI CRWTRTGTKS TLPISETGGG TPELRCGKHQ 910 920 930 AVGTSPQRAA AQDSEQESAG MEGRDTHRLP RAL

CATM prediction

The sequence of PERT_HUMAN returned 6 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
        850       860       870  
 +    |    +    |    +    |    		 (Å) (°) (Å) (°)  
Model 1 LARVTWISMSLAALLIGGFAGLTSTVICRWT 6.4 -48.0° 0.5 46.9 -38.7
Model 2 LARVTWISMSLAALLIGGFAGLTSTVICRWT 7.1 -43.0° 3.5 49.8 -31.3
Model 3 LARVTWISMSLAALLIGGFAGLTSTVICRWT 7.1 -47.7° 5.1 38.7 -20.1
Model 4 LARVTWISMSLAALLIGGFAGLTSTVICRWT 6.8 -55.1° 1.6 60.0 -18.6
Model 5 LARVTWISMSLAALLIGGFAGLTSTVICRWT 7.1 -40.1° 2.7 33.9 -18.1
Model 6 LARVTWISMSLAALLIGGFAGLTSTVICRWT 7.3 -32.0° 2.9 49.9 -15.7
Prolines* LPRVTWISMSLAALLIGGFAGLTSTVICRWT          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 857, LEU, HA A, 856, ALA, O 3.19Å
B, 860, GLY, HA1 A, 857, LEU, O 2.57Å
B, 861, GLY, HA2 A, 860, GLY, O 2.44Å
B, 864, GLY, HA1 A, 861, GLY, O 3.29Å
A, 857, LEU, HA B, 856, ALA, O 3.19Å
A, 860, GLY, HA1 B, 857, LEU, O 2.57Å
A, 861, GLY, HA2 B, 860, GLY, O 2.44Å
A, 864, GLY, HA1 B, 861, GLY, O 3.29Å