Uroplakin-3a

UniProt data

UniProt name: UPK3A_HUMAN (O75631)

Gene name: UPK3A; Synonyms=UPK3

Organism: Homo sapiens (Human).

Function: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence (By similarity). {ECO:0000250}.

Subcellular location: Endoplasmic reticulum membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}. Note=Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER). {ECO:0000250}.

Transmembrane domains:

  1. 208-235, 28 AA, Helical. {ECO:0000255}.

Sequence: 287 AA

10 20 30 40 50 60 MPPLWALLAL GCLRFGSAVN LQPQLASVTF ATNNPTLTTV ALEKPLCMFD SKEALTGTHE 70 80 90 100 110 120 VYLYVLVDSA ISRNASVQDS TNTPLGSTFL QTEGGRTGPY KAVAFDLIPC SDLPSLDAIG 130 140 150 160 170 180 DVSKASQILN AYLVRVGANG TCLWDPNFQG LCNAPLSAAT EYRFKYVLVN MSTGLVEDQT 190 200 210 220 230 240 LWSDPIRTNQ LTPYSTIDTW PGRRSGGMIV ITSILGSLPF FLLVGFAGAI ALSLVDMGSS 250 260 270 280 DGETTHDSQI TQEAVPKSLG ASESSYTSVN RGPPLDRAEV YSSKLQD

CATM prediction

The sequence of UPK3A_HUMAN returned 3 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       210       220       230      
+    |    +    |    +    |    +   		 (Å) (°) (Å) (°)  
Model 1 SGGMIVITSILGSLAFFLLVGFAGAIALSLVDMG 7.1 -53.1° 3.2 34.0 -27.2
Model 2 SGGMIVITSILGSLAFFLLVGFAGAIALSLVDMG 7.3 -42.0° 1.4 45.4 -21.2
Model 3 SGGMIVITSILGSLAFFLLVGFAGAIALSLVDMG 6.8 -55.0° 1.5 44.8 -13.2
Prolines* SGGMIVITSILGSLPFFLLVGFAGAIALSLVDMG          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 225, GLY, HA2 A, 224, VAL, O 3.25Å
B, 228, GLY, HA1 A, 225, GLY, O 3.13Å
A, 225, GLY, HA2 B, 224, VAL, O 3.25Å
A, 228, GLY, HA1 B, 225, GLY, O 3.13Å