Beta-1,4-galactosyltransferase 3

UniProt data

UniProt name: B4GT3_HUMAN (O60512)

Gene name: B4GALT3

Organism: Homo sapiens (Human).

Function: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Subcellular location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Trans cisternae of Golgi stack.

Transmembrane domains:

  1. 11-31, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 393 AA

10 20 30 40 50 60 MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DQGPTFDYSH PRDVYSNLSH 70 80 90 100 110 120 LPGAPGGPPA PQGLPYCPER SPLLVGPVSV SFSPVPSLAE IVERNPRVEP GGRYRPAGCE 130 140 150 160 170 180 PRSRTAIIVP HRAREHHLRL LLYHLHPFLQ RQQLAYGIYV IHQAGNGTFN RAKLLNVGVR 190 200 210 220 230 240 EALRDEEWDC LFLHDVDLLP ENDHNLYVCD PRGPRHVAVA MNKFGYSLPY PQYFGGVSAL 250 260 270 280 290 300 TPDQYLKMNG FPNEYWGWGG EDDDIATRVR LAGMKISRPP TSVGHYKMVK HRGDKGNEEN 310 320 330 340 350 360 PHRFDLLVRT QNSWTQDGMN SLTYQLLARE LGPLYTNITA DIGTDPRGPR APSGPRYPPG 370 380 390 SSQAFRQEML QRRPPARPGP LSTANHTALR GSH

CATM prediction

The sequence of B4GT3_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
    10        20        30   
  |    +    |    +    |    		 (Å) (°) (Å) (°)  
Model 1 RACTLALLVGSQLAVMMYLSLGGFRSL 7.1 -37.8° 5.7 33.9 -28.8
Model 2 RACTLALLVGSQLAVMMYLSLGGFRSL 7.2 -51.2° 6.0 45.0 -0.7
Prolines* RPCTLALLVGSQLAVMMYLSLGGFRSL          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 14, LEU, HA A, 13, ALA, O 2.93Å
B, 17, GLY, HA1 A, 14, LEU, O 3.27Å
A, 14, LEU, HA B, 13, ALA, O 2.93Å
A, 17, GLY, HA1 B, 14, LEU, O 3.27Å
A, 22, VAL, HA B, 25, TYR, OH 2.32Å