Heparan-sulfate 6-O-sulfotransferase 1

UniProt data

UniProt name: H6ST1_HUMAN (O60243)

Gene name: HS6ST1; Synonyms=HS6ST

Organism: Homo sapiens (Human).

Function: 6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Critical for normal neuronal development where it may play a role in neuron branching. May also play a role in limb development. May prefer iduronic acid. {ECO:0000269|PubMed:21700882}.

Subcellular location: Membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.

Transmembrane domains:

  1. 18-37, 20 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 411 AA

10 20 30 40 50 60 MRRRRAGGRT MVERASKFVL VVAGSVCFML ILYQYAGPGL SLGAPGGRAP PDDLDLFPTP 70 80 90 100 110 120 DPHYEKKYYF PVRELERSLR FDMKGDDVIV FLHIQKTGGT TFGRHLVQNV RLEVPCDCRP 130 140 150 160 170 180 GQKKCTCYRP NRRETWLFSR FSTGWSCGLH ADWTELTNCV PGVLDRRDSA ALRTPRKFYY 190 200 210 220 230 240 ITLLRDPVSR YLSEWRHVQR GATWKTSLHM CDGRTPTPEE LPPCYEGTDW SGCTLQEFMD 250 260 270 280 290 300 CPYNLANNRQ VRMLADLSLV GCYNLSFIPE GKRAQLLLES AKKNLRGMAF FGLTEFQRKT 310 320 330 340 350 360 QYLFERTFNL KFIRPFMQYN STRAGGVEVD EDTIRRIEEL NDLDMQLYDY AKDLFQQRYQ 370 380 390 400 410 YKRQLERREQ RLRSREERLL HRAKEALPRE DADEPGRVPT EDYMSHIIEK W

CATM prediction

The sequence of H6ST1_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
       20        30        4
+    |    +    |    +    |		 (Å) (°) (Å) (°)  
Model 1 ASKFVLVVAGSVCFMLILYQYAGAGL 7.1 -53.0° 2.0 45.0 -5.6
Prolines* ASKFVLVVAGSVCFMLILYQYAGPGL          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 21, VAL, HA A, 20, LEU, O 3.40Å
B, 24, GLY, HA1 A, 21, VAL, O 3.04Å
A, 21, VAL, HA B, 20, LEU, O 3.40Å
A, 24, GLY, HA1 B, 21, VAL, O 3.04Å