Fatty-acid amide hydrolase 1

UniProt data

UniProt name: FAAH1_HUMAN (O00519)

Gene name: FAAH; Synonyms=FAAH1

Organism: Homo sapiens (Human).

Function: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. {ECO:0000269|PubMed:17015445}.

Subcellular location: Endomembrane system {ECO:0000269|PubMed:17015445}; Single-pass membrane protein {ECO:0000269|PubMed:17015445}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:17015445}. Note=Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.

Transmembrane domains:

  1. 9-29, 21 AA, Helical. {ECO:0000255}.

Sequence: 579 AA

10 20 30 40 50 60 MVQYELWAAL PGASGVALAC CFVAAAVALR WSGRRTARGA VVRARQRQRA GLENMDRAAQ 70 80 90 100 110 120 RFRLQNPDLD SEALLALPLP QLVQKLHSRE LAPEAVLFTY VGKAWEVNKG TNCVTSYLAD 130 140 150 160 170 180 CETQLSQAPR QGLLYGVPVS LKECFTYKGQ DSTLGLSLNE GVPAECDSVV VHVLKLQGAV 190 200 210 220 230 240 PFVHTNVPQS MFSYDCSNPL FGQTVNPWKS SKSPGGSSGG EGALIGSGGS PLGLGTDIGG 250 260 270 280 290 300 SIRFPSSFCG ICGLKPTGNR LSKSGLKGCV YGQEAVRLSV GPMARDVESL ALCLRALLCE 310 320 330 340 350 360 DMFRLDPTVP PLPFREEVYT SSQPLRVGYY ETDNYTMPSP AMRRAVLETK QSLEAAGHTL 370 380 390 400 410 420 VPFLPSNIPH ALETLSTGGL FSDGGHTFLQ NFKGDFVDPC LGDLVSILKL PQWLKGLLAF 430 440 450 460 470 480 LVKPLLPRLS AFLSNMKSRS AGKLWELQHE IEVYRKTVIA QWRALDLDVV LTPMLAPALD 490 500 510 520 530 540 LNAPGRATGA VSYTMLYNCL DFPAGVVPVT TVTAEDEAQM EHYRGYFGDI WDKMLQKGMK 550 560 570 KSVGLPVAVQ CVALPWQEEL CLRFMREVER LMTPEKQSS

CATM prediction

The sequence of FAAH1_HUMAN returned 3 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
      10        20        30 
    |    +    |    +    |  		 (Å) (°) (Å) (°)  
Model 1 LWAALAGASGVALACCFVAAAVALRWS 6.8 -46.0° 2.7 47.0 -27.7
Model 2 LWAALAGASGVALACCFVAAAVALRWS 7.3 -29.0° 2.5 37.0 -14.0
Model 3 LWAALAGASGVALACCFVAAAVALRWS 6.8 -46.0° 4.7 38.0 -14.0
Prolines* LWAALPGASGVALACCFVAAAVALRWS          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 12, GLY, HA2 A, 11, ALA, O 2.78Å
B, 15, GLY, HA1 A, 12, GLY, O 2.74Å
B, 16, VAL, HA A, 15, GLY, O 3.37Å
A, 12, GLY, HA2 B, 11, ALA, O 2.78Å
A, 15, GLY, HA1 B, 12, GLY, O 2.74Å
A, 16, VAL, HA B, 15, GLY, O 3.37Å