Golgi integral membrane protein 4

UniProt data

UniProt name: GOLI4_HUMAN (O00461)

Gene name: GOLIM4; Synonyms=GIMPC, GOLPH4, GPP130

Organism: Homo sapiens (Human).

Function: Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi. {ECO:0000269|PubMed:15331763}.

Subcellular location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Endosome membrane; Single- pass type II membrane protein. Membrane {ECO:0000305}; Lipid- anchor {ECO:0000305}. Note=Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.

Transmembrane domains:

  1. 13-33, 21 AA, Helical; Signal-anchor for type II membrane protein. {ECO:0000255}.

Sequence: 696 AA

10 20 30 40 50 60 MGNGMCSRKQ KRIFQTLLLL TVVFGFLYGA MLYYELQTQL RKAEAVALKY QQHQESLSAQ 70 80 90 100 110 120 LQVVYEHRSR LEKSLQKERL EHKKAKEDFL VYKLEAQETL NKGRQDSNSR YSALNVQHQM 130 140 150 160 170 180 LKSQHEELKK QHSDLEEEHR KQGEDFSRTF NDHKQKYLQL QQEKEQELSK LKETVYNLRE 190 200 210 220 230 240 ENRQLRKAHQ DIHTQLQDVK QQHKNLLSEH EQLVVTLEDH KSALAAAQTQ VAEYKQLKDT 250 260 270 280 290 300 LNRIPSLRKP DPAEQQNVTQ VAHSPQGYNT AREKPTREVQ EVSRNNDVWQ NHEAVPGRAE 310 320 330 340 350 360 DTKLYAPTHK EAEFQAPPEP IQQEVERREP EEHQVEEEHR KALEEEEMEQ VGQAEHLEEE 370 380 390 400 410 420 HDPSPEEQDR EWKEQHEQRE AANLLEGHAR AEVYPSAKPM IKFQSPYEEQ LEQQRLAVQQ 430 440 450 460 470 480 VEEAQQLREH QEALHQQRLQ GHLLRQQEQQ QQQVAREMAL QRQAELEEGR PQHQEQLRQQ 490 500 510 520 530 540 AHYDAMDNDI VQGAEDQGIQ GEEGAYERDN QHQDEAEGDP GNRHEPREQG PREADPESEA 550 560 570 580 590 600 DRAAVEDINP ADDPNNQGED EFEEAEQVRE ENLPDENEEQ KQSNQKQENT EVEEHLVMAG 610 620 630 640 650 660 NPDQQEDNVD EQYQEEAEEE VQEDLTEEKK RELEHNAEET YGENDENTDD KNNDGEEQEV 670 680 690 RDDNRPKGRE EHYEEEEEEE EDGAAVAEKS HRRAEM

CATM prediction

The sequence of GOLI4_HUMAN returned 2 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
  10        20        30     
|    +    |    +    |    + 		 (Å) (°) (Å) (°)  
Model 1 QKRIFQTLLLLTVVFGFLYGAMLYYEL 7.0 -52.9° 1.9 45.3 -20.2
Model 2 QKRIFQTLLLLTVVFGFLYGAMLYYEL 6.7 -55.0° 1.5 45.2 -18.9

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 25, GLY, HA1 A, 22, VAL, O 3.27Å
B, 26, PHE, HA A, 25, GLY, O 3.15Å
B, 30, ALA, HA A, 33, TYR, OH 2.75Å
A, 25, GLY, HA1 B, 22, VAL, O 3.27Å
A, 26, PHE, HA B, 25, GLY, O 3.15Å
A, 30, ALA, HA B, 33, TYR, OH 3.00Å