HEPACAM family member 2

UniProt data

UniProt name: HECA2_HUMAN (A8MVW5)

Gene name: HEPACAM2; Synonyms=MIKI; ORFNames=UNQ305/PRO346

Organism: Homo sapiens (Human).

Function: Required during prometaphase for centrosome maturation. Following poly-ADP-ribosylation (PARsylation) by TNKS, translocates from the Golgi apparatus to mitotic centrosomes and plays a key role in the formation of robust microtubules for prompt movement of chromosomes: anchors AKAP9/CG-NAP, a scaffold protein of the gamma-tubulin ring complex and promotes centrosome maturation. {ECO:0000269|PubMed:22864114}.

Subcellular location: Golgi apparatus membrane {ECO:0000269|PubMed:19358830, ECO:0000269|PubMed:22864114}; Single-pass type I membrane protein {ECO:0000255}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:19358830, ECO:0000269|PubMed:22864114}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:19358830, ECO:0000269|PubMed:22864114}. Midbody {ECO:0000269|PubMed:22864114}. Note=In interphase, localizes to the Golgi apparatus. Localizes to centrosomes and spindles during prophase, prometaphase, and metaphase of mitosis, and to midbodies at telophase. Translocation to mitotic centrosomes is the result of poly-ADP-ribosylation (PARsylation). {ECO:0000269|PubMed:19358830, ECO:0000269|PubMed:22864114}.

Transmembrane domains:

  1. 352-372, 21 AA, Helical. {ECO:0000255}.

Sequence: 462 AA

10 20 30 40 50 60 MGQDAFMEPF GDTLGVFQCK IYLLLFGACS GLKVTVPSHT VHGVRGQALY LPVHYGFHTP 70 80 90 100 110 120 ASDIQIIWLF ERPHTMPKYL LGSVNKSVVP DLEYQHKFTM MPPNASLLIN PLQFPDEGNY 130 140 150 160 170 180 IVKVNIQGNG TLSASQKIQV TVDDPVTKPV VQIHPPSGAV EYVGNMTLTC HVEGGTRLAY 190 200 210 220 230 240 QWLKNGRPVH TSSTYSFSPQ NNTLHIAPVT KEDIGNYSCL VRNPVSEMES DIIMPIIYYG 250 260 270 280 290 300 PYGLQVNSDK GLKVGEVFTV DLGEAILFDC SADSHPPNTY SWIRRTDNTT YIIKHGPRLE 310 320 330 340 350 360 VASEKVAQKT MDYVCCAYNN ITGRQDETHF TVIITSVGLE KLAQKGKSLS PLASITGISL 370 380 390 400 410 420 FLIISMCLLF LWKKYQPYKV IKQKLEGRPE TEYRKAQTFS GHEDALDDFG IYEFVAFPDV 430 440 450 460 SGVSRIPSRS VPASDCVSGQ DLHSTVYEVI QHIPAQQQDH PE

CATM prediction

The sequence of HECA2_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
   350       360       370   
 |    +    |    +    |    +		 (Å) (°) (Å) (°)  
Model 1 LSALASITGISLFLIISMCLLFLWKKY 6.9 -51.0° 4.4 40.1 -18.2
Prolines* LSPLASITGISLFLIISMCLLFLWKKY          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


Click on the image to enlarge

Download model 1 structure: PDB, PyMOL session

List of hydrogen bonds:

Donor Acceptor Distance
B, 354, SER, HA A, 353, ALA, O 2.99Å
B, 357, GLY, HA1 A, 354, SER, O 3.28Å
A, 354, SER, HA B, 353, ALA, O 2.99Å
A, 357, GLY, HA1 B, 354, SER, O 3.28Å