Metalloprotease TIKI2

UniProt data

UniProt name: TIKI2_HUMAN (A6NFA1)

Gene name: TRABD2B; Synonyms=HKAT, TIKI2

Organism: Homo sapiens (Human).

Function: Metalloprotease that acts as a negative regulator of the Wnt signaling pathway by mediating the cleavage of the 8 N- terminal residues of a subset of Wnt proteins. Following cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation. Able to cleave WNT3A, WNT5, but not WNT11. Required for head formation. {ECO:0000269|PubMed:22726442}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:22726442}; Single-pass type I membrane protein {ECO:0000269|PubMed:22726442}.

Transmembrane domains:

  1. 495-515, 21 AA, Helical. {ECO:0000255}.

Sequence: 517 AA

10 20 30 40 50 60 MHAALAGPLL AALLATARAR PQPPDGGQCR PPGSQRDLNS FLWTIRRDPP AYLFGTIHVP 70 80 90 100 110 120 YTRVWDFIPD NSKAAFQAST RVYFELDLTD PYTISALASC QLLPHGENLQ DVLPHELYWR 130 140 150 160 170 180 LKRHLDYVKL MMPSWMTPAQ RGKGLYADYL FNAIAGNWER KRPVWVMLMV NSLTERDVRF 190 200 210 220 230 240 RGVPVLDLYL AQQAEKMKKT TGAVEQVEEQ CHPLNNGLNF SQVLFALNQT LLQQESVRAG 250 260 270 280 290 300 SLQASYTTED LIKHYNCGDL SAVIFNHDTS QLPNFINTTL PPHEQVTAQE IDSYFRQELI 310 320 330 340 350 360 YKRNERMGKR VMALLRENED KICFFAFGAG HFLGNNTVID ILRQAGLEVD HTPAGQAIHS 370 380 390 400 410 420 PAPQSPAPSP EGTSTSPAPV TPAAAVPEAP SVTPTAPPED EDPALSPHLL LPDSLSQLEE 430 440 450 460 470 480 FGRQRKWHKR QSTHQRPRQF NDLWVRIEDS TTASPPPLPL QPTHSSGTAK PPFQLSDQLQ 490 500 510 QQDPPGPASS SAPTLGLLPA IATTIAVCFL LHSLGPS

CATM prediction

The sequence of TIKI2_HUMAN returned 1 potential models

The positions at the dimer's interface are highlighted in yellow. The position at the crossing point is highlighted in orange.

# Sequence and interface d θ Z' ω' Score
          500       510     
   +    |    +    |    +  		 (Å) (°) (Å) (°)  
Model 1 AATLGLLAAIATTIAVCFLLHSLGAS 7.3 -35.0° 5.0 35.0 -2.1
Prolines* APTLGLLPAIATTIAVCFLLHSLGPS          

* Note: this sequence contains Pro residues. CATM substituted them with Ala during the modeling. The positions are marked.

Click on the model number to visualize its details below.

MODEL 1 DETAILS

Precited structure:


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Download model 1 structure: PDB, PyMOL session